Product Name :
PABPN1 Recombinant Rabbit Monoclonal Antibody [JM11-28]
Predicted band size :
Observed band size :
Synonyms:
Nuclear poly(A)-binding protein 1 antibody OPMD antibody PAB2 antibody PABII antibody PABP 2 antibody pABP-2 antibody PABP2 antibody PABP2_HUMAN antibody PABPII antibody Pabpn1 antibody 展开
Function :
Involved in the 3′-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within Human PABPN1 aa 4-51 / 306.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Coupling :
unconjugated
Clone number:
JM11-28
RRID:
AB_3070421
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Cl-PEG2-acid Purity Aliquot store at -20℃ or -80℃.BuyN-Hydroxysulfosuccinimide (sodium) Avoid repeated freeze / thaw cycles.PMID:33477174
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 33 kDa
Positive control :
MCF-7 cell lysate, SH-SY5Y cell lysate, rat skin tissue lysate, AGS, SW480, mouse testis tissue, Hela.