Product Name :
Phospho-eIF4B (S406) Recombinant Rabbit Monoclonal Antibody [JE63-80]
Predicted band size :
69 kDa
Observed band size :
80 kDa
Synonyms:
2310046H11Rik antibody AL024095 antibody C85189 antibody EIF 4B antibody eIF-4B antibody EIF4B antibody Eukaryotic initiation factor 4B antibody Eukaryotic translation initiation factor 4B antibody IF4B_HUMAN antibody PRO1843 antibody
Function :
Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation.6-Amino-2-cyanobenzothiazole Purity In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens, and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide corresponding to residues surrounding Ser406 of human eIF4B protein.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IHC-P, FC
Coupling :
unconjugated
Clone number:
JE63-80
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.2-(3-Fluoro-2-methoxyphenyl)acetic acid Chemscene Aliquot store at -20℃.PMID:33661228 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 69 kDa
Positive control :
293T cell lysate, 293T treated with 100nM Calyculin A for 15 minutes cell lysate, HeLa cell lysate, HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, Jurkat cell lysate, Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, human breast cancer tissue, Jurkat, NIH/3T3, PC-12.