Product Name :
Phospho-Chk1 (S317) Recombinant Rabbit Monoclonal Antibody [PSH03-84]
Predicted band size :
54 kDa
Observed band size :
54 kDa
Synonyms:
C85740 antibody Cell cycle checkpoint kinase antibody Checkpoint , S. pombe, homolog of, 1 antibody Checkpoint kinase 1 antibody Checkpoint kinase 1 homolog (S. pombe) antibody CHEK 1 antibody Chek1 antibody Chk 1 antibody Chk1 antibody CHK1 checkpoint homolog (S. pombe) antibody 展开
Function :
Checkpoint kinases (Chks) are protein kinases that are involved in cell cycle control. Two checkpoint kinase subtypes have been identified, Chk1 and Chk2. Chk1 is a central component of genome surveillance pathways and is a key regulator of the cell cycle and cell survival. Chk1 is required for the initiation of DNA damage checkpoints and has recently been shown to play a role in the normal (unperturbed) cell cycle.73286-71-2 manufacturer Chk1 impacts various stages of the cell cycle including the S phase, G2/M transition and M phaase. In addition to mediating cell cycle checkpoints, Chk1 also contributes to DNA repair processes, gene transcription, egg production, embryo development, cellular responses to HIV infection and somatic cell viability.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser317 of Human Chk1.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P
Coupling :
unconjugated
Clone number:
PSH03-84
RRID:
AB_3096843
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.457613-78-4 Chemscene Aliquot store at -20℃.PMID:33742371 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 54 kDa
Positive control :
HeLa cell lysate, HeLa treated with UV for 1 hour cell lysate, C6 cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, HeLa cells treated with or without UV for 40 minutes, NIH/3T3 cell lysate, NIH/3T3 treated with UV for 40 minutes add 1mM Sodium orthovanadate and recovery for 30 minutes cell lysate, L-929 treated with UV for 3 hours cell lysate.